rabbit anti prdx2  (Proteintech)

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The images of western blotting. Distinct single bands were detected in lysate from HeLa or Re12 by western blotting using anti-human peroxiredoxin (PRDX) 1 or 2 antibodies. Molecular weight of the bands was approximately 22 kDa. β-actin as a loading control was detected at approximately 45 kDa in both HeLa and Re12.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Western Blot, Molecular Weight, Control

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: Staining properties of peroxiredoxin (PRDX) 1 in normal canine tissues. ( A ) Squamous cells in the epidermis. The nuclei of squamous cells located in the basal and spinous layers were strongly positive for PRDX1 and the cytoplasm was weakly positive. However, squamous cells in the granular layer were negative for PRDX1. Bar, 20 µm. ( B ) Squamous cells of hair follicles (arrows) and sebaceous glands (arrowhead). The nuclei of squamous cells were positive for PRDX1 and the cytoplasm was weakly positive. Almost all sebaceous gland cells were positive for PRDX1 expression. Bar, 50 µm. ( C ) Apocrine sweat glands. Glandular epithelial cells were positive for PRDX1, but myoepithelial cells surrounding the glands were negative. Bar, 20 µm. ( D ) Macrophages in the dermis. The nucleus and cytoplasm of macrophages were labeled with PRDX1 (arrow). Bar, 20 µm. ( E ) Vascular vessels in the dermis. Almost all the endothelial cells in the vascular vessels were negative for PRDX1. Bar, 20 µm. ( F ) Fibroblasts in the dermis. Fibroblasts were negative to positive for PRDX1. Bar, 20 µm. ( G ) Striated cutaneous muscle. The nucleus and cytoplasm of striated muscle were negative for PRDX1 expression. Bar, 50 µm. ( H ) Neutrophil in the spleen. Both of the nucleus and cytoplasm of neutrophils were negative for PRDX1 (arrow). Bar, 20 µm. ( I ) Lymphocytes in the lymphoid follicles of the spleen. The cytoplasm of the lymphocytes was positive for PRDX1. Bar, 20 µm. ( J ) Erythroblasts in the spleen. Erythroblasts were positive for PRDX1 expression. Bar, 20 µm. ( K ) Endothelial cells of splenic sinusoids. The nuclei of the endothelial cells of the splenic sinusoid were negative to weakly positive for PRDX1. Bar, 20 µm.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Staining, Expressing, Labeling

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: Staining properties of peroxiredoxin (PRDX) 2 in normal canine tissues. ( A ) Squamous cells in the epidermis. The nuclei of squamous cells located in the basal and spinous layers were strongly positive for PRDX2 and the cytoplasm was weakly positive; however, squamous cells in the granular layer were negative for PRDX2. Bar, 20 µm. ( B ) Squamous cells of hair follicles and sebaceous glands. Nuclei of squamous cells were negative to positive for PRDX2 and the cytoplasm was weakly positive (arrow). Immature and mature sebaceous gland cells were positive for PRDX2 (arrowheads). Bar, 50 µm. ( C ) Apocrine sweat glands. The glandular epithelial cells were positive for PRDX2 expression. Myoepithelial cells surrounding the glands were negative to weakly positive for PRDX2 expression. Bar, 20 µm. ( D ) Macrophages in the dermis. Macrophages were negative to weakly positive for PRDX2 (arrow). Bar, 20 µm. ( E ) Microvessels in the dermis. Almost all endothelial cells in microvessels were negative for PRDX2. Bar, 20 µm. ( F ) Fibroblasts in the dermis. Fibroblasts were negative to weakly positive for PRDX2 although some erythrocytes were strongly positive for PRDX2. Bar, 20 µm. ( G ) Striated cutaneous muscle. The nucleus and cytoplasm of striated muscles were negative for PRDX2 expression. Bar, 50 µm. ( H ) Neutrophil in the spleen. The nucleus and cytoplasm of neutrophils were negative for PRDX2 (arrow). Bar, 20 µm. ( I ) Lymphocytes in lymphoid follicles of the spleen. Almost all lymphocytes were negative for PRDX2. Bar, 20 µm. ( J ) Erythroblasts in the spleen. The cytoplasm of these cells was found to be strongly positive for PRDX2. Bar, 20 µm. ( K ) Endothelial cells of splenic sinusoids. Endothelial cells of the splenic sinusoid were negative for PRDX2. Bar, 20 µm.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Staining, Expressing, Muscles

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The staining properties of peroxiredoxin (PRDX) 1 and 2 in canine normal tissues

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Staining

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The staining properties of peroxiredoxin (PRDX) 1 and 2 in the newly formed vascular vessels of canine granulation tissues. ( A ) The nucleus and cytoplasm of endothelial cells in angiogenic region were positive for PRDX1 (arrows). Bar, 50 µm. ( B ) The nucleus and cytoplasm of endothelial cells in angiogenic region were positive for PRDX2 (arrows). Bar, 50 µm. ( C ) The nucleus and cytoplasm of endothelial cells in resting region were negative for PRDX1 (arrows). Bar, 50 µm. ( D ) The nucleus and cytoplasm of endothelial cells in resting region were negative for PRDX2 (arrows). Bar, 50 µm.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Staining

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The expression levels of peroxiredoxin (PRDX) 1 and 2 in canine hemangioma (HA) and hemangiosarcoma (HSA) tissues. ( A ) Immunohistochemical (IHC) staining image for PRDX1 expression in canine HA. Macrophages in HA tissues were positive for PRDX1 (arrow). HA cells were negative for PRDX1. IHC score of this sample is 0.00. Bar, 50 µm. ( B ) IHC staining image for PRDX2 in canine HA. Some erythrocytes were positive for PRDX2 (arrows). HA cells were negative for PRDX2. IHC score of this sample is 0.00. Bar, 50 µm. ( C ) IHC staining image for PRDX1 in canine HSA. Macrophages (arrow) and neutrophils (arrowhead) were observed as positive and negative control for PRDX1, respectively. HSA cells were positive for PRDX1. IHC score of this sample is 12.00. Bar, 50 µm. ( D ) IHC staining image for PRDX2 in canine HSA. Some erythrocytes stained positively (arrow) and neutrophils (arrowhead) were used as positive and negative control for PRDX2, respectively. HSA cells were positive for PRDX2. IHC score of this sample is 12.00. Bar, 50 µm. ( E) The box-and-whisker plot of IHC scores of PRDX1 in canine HA and HSA. IHC score of PRDX1 in HSA was significantly higher than that in HA. Bar crossing the box-and-whisker plot expresses the sample average. ( F ) The box-and-whisker plot of IHC scores of PRDX2 in canine HA and HSA. IHC score of PRDX2 in HSA was significantly higher than that in HA. Bar crossing the box-and-whisker plot expresses the sample average. * P <0.05.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Negative Control, Staining, Whisker Assay

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The expression of peroxiredoxin (PRDX) 1 and 2 in histopathological subtypes of canine hemangiosarcoma (HSA). ( A ) The box-and-whisker plot of the immunohistochemical (IHC) scores of PRDX1 in each histopathological subtypes of canine HSA. There were no significant differences in the expressions of PRDX1 among three subtypes of HSA. ( B ) The box-and-whisker plot of IHC scores of PRDX2 in each histopathological subtypes of canine HSA. There were no significant differences in the expressions of PRDX2 among three subtypes. Bar crossing the box-and-whisker plot expresses the sample average.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Expressing, Whisker Assay, Immunohistochemical staining

Journal: The Journal of Veterinary Medical Science

Article Title: The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

doi: 10.1292/jvms.22-0102

Figure Lengend Snippet: The expression of peroxiredoxin (PRDX) 1 and 2 of canine hemangiosarcoma (HSA) between spleen (n=27) and skin (n=27). ( A ) The box-and-whisker plot of immunohistochemical (IHC) scores of PRDX1 of canine HSA in spleen and skin. There were no significant differences in the expressions of PRDX1 between spleen and skin. ( B ) The box-and-whisker plot of IHC scores of PRDX2 of canine HSA in spleen and skin. There were no significant differences in the expressions of PRDX2 between spleen and skin. Bar crossing the box-and-whisker plot expresses the sample average.

Article Snippet: After blocking, the sections were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal antibody to PRDX1 (1:500, ab228780; Abcam), rabbit polyclonal antibody to PRDX2 (1:1,000, ab109367; Abcam), and mouse monoclonal antibody against CD31.

Techniques: Expressing, Whisker Assay, Immunohistochemical staining

Journal: Cell reports

Article Title: Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy

doi: 10.1016/j.celrep.2022.110475

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-Peroirdoxin 2 (PRDX2) (1:1000) , ProteinTech , Cat# 10545–2-AP; RRID: AB_2168202.

Techniques: Transduction, Recombinant, Western Blot, Multiple Displacement Amplification, Reverse Transcription, Bradford Protein Assay, DNA Purification, Gene Expression, Software

Journal: Journal of Virology

Article Title: APOBEC3G-Regulated Host Factors Interfere with Measles Virus Replication: Role of REDD1 and Mammalian TORC1 Inhibition

doi: 10.1128/JVI.00835-18

Figure Lengend Snippet: Microarray analysis reveals A3G-upregulated and -downregulated genes. (A) Box plots of log2 values for quantile-quantile normalized probe signal intensities. Total RNA was isolated independently two times each from control Vero-023 cells and A3G-expressing Vero (Vero-A3G) cells and labeled. Bars 1 and 3, RNAs from Vero-023 control cells; bars 2 and 4, RNAs from Vero-A3G cells. The probes were hybridized to Gene Chip rhesus macaque genome arrays (Affymetrix) according to the manufacturer's instructions. (B) MA plot (intensity log2 fold change [M] plotted against the average log2 intensity [A]) for the comparison between treated and untreated cells. Mean intensities of both groups (x axis) were plotted against log2 fold change (y axis). Blue circles around dots show the first 15 genes with the lowest P values. (C) Numbers of all probe sets, of significantly (adjusted P values of <0.05) up- and downregulated probe sets, and of more than 2-fold-up- and downregulated probe sets. (D) List of the best up- and downregulated genes, with the alteration (ratio) of transcript expression and significance (adjusted P values) as determined by the four microarrays. (E) Total RNA was isolated from control (Vero-023) and A3G-expressing (Vero-A3G) cells. Four amounts of RNA (4, 2, 1, and 0.5 μg) were reverse transcribed into cDNA. Four microliters of each of cDNA product was amplified by PCR and analyzed on 1% agarose gels. (F) Protein lysates of Vero-023 and Vero-A3G cells were separated by 10% SDS-PAGE, blotted to nitrocellulose, incubated with antibodies to REDD1, KDELR2, MOSC2, ACY1, and PRDX2 (and GAPDH as a control), and visualized using the ECL system. Quantifications of the Western blots normalized to the value for Vero-023 cells are shown below each blot.

Article Snippet: The following unlabeled or labeled primary antibodies were used in immunoblotting and flow cytometry: rabbit monoclonal anti-ACY1 (Epitomics, 5879-1), rabbit polyclonal anti-MOSC2 (Epitomics, T3362), rabbit anti-DDIT4/REDD1 (ProteinTech, 10638-1-AP), rabbit anti-PRDX2 (Sigma, SAB2101878), rabbit anti-KDELR2 (Sigma, SAB1401554), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) (Santa Cruz, sc-25778), rabbit anti-phospho-p70 S6 kinase Thr 389 (Cell Signaling, 9205), rabbit anti-p70S6 kinase (Cell Signaling, 9202), phycoerythrin (PE)–anti-human CD3 (BioLegend, 300408), allophycocyanin (APC)–anti-human CD69 (BioLegend, 310910), and rabbit polyclonal anti-A3G (a kind gift from M. Malim, King's College, London, United Kingdom).

Techniques: Microarray, Isolation, Expressing, Labeling, Amplification, SDS Page, Incubation, Western Blot

Journal: Journal of Virology

Article Title: APOBEC3G-Regulated Host Factors Interfere with Measles Virus Replication: Role of REDD1 and Mammalian TORC1 Inhibition

doi: 10.1128/JVI.00835-18

Figure Lengend Snippet: Functional characterization of A3G-downregulated genes. (A) Vero-023 cells were transduced with lentiviral vectors expressing shRNAs against ACY1, MOSC2, PRDX2, and TXNIP, and the transduction efficiency was confirmed by detection of vector-mediated DsRed expression by flow cytometry. (B) The shRNA-mediated knockdown was confirmed at the RNA level by RT-PCR. Total RNAs from transduced cells and Vero-023 control cells were prepared, and four amounts of RNA (4, 2, 1, and 0.5 μg) were reverse transcribed into cDNA. Four microliters of each of cDNA product was amplified by PCR. (C) Vero-023, Vero-A3G, and shRNA-transduced Vero-023 cells (as indicated) were infected with MV-eGFP at an MOI of 0.1, and viral titers produced by infected cultures were determined after 48 h. Data from three independent experiments were normalized to values for Vero-023 cells and are presented as percentages of control values (n = 3). Virus titers were significantly reduced in A3G-expressing cells (bar 2) and in MOSC2 shRNA-expressing cells (bar 5) compared to those in Vero-023 cells (*, P < 0.05; **, P < 0.01 [Student's t test]).

Article Snippet: The following unlabeled or labeled primary antibodies were used in immunoblotting and flow cytometry: rabbit monoclonal anti-ACY1 (Epitomics, 5879-1), rabbit polyclonal anti-MOSC2 (Epitomics, T3362), rabbit anti-DDIT4/REDD1 (ProteinTech, 10638-1-AP), rabbit anti-PRDX2 (Sigma, SAB2101878), rabbit anti-KDELR2 (Sigma, SAB1401554), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) (Santa Cruz, sc-25778), rabbit anti-phospho-p70 S6 kinase Thr 389 (Cell Signaling, 9205), rabbit anti-p70S6 kinase (Cell Signaling, 9202), phycoerythrin (PE)–anti-human CD3 (BioLegend, 300408), allophycocyanin (APC)–anti-human CD69 (BioLegend, 310910), and rabbit polyclonal anti-A3G (a kind gift from M. Malim, King's College, London, United Kingdom).

Techniques: Functional Assay, Transduction, Expressing, Plasmid Preparation, Flow Cytometry, shRNA, Reverse Transcription Polymerase Chain Reaction, Amplification, Infection, Produced